Integrated multi-omics unveil the impact of H-phosphinic analogs of glutamate and α-ketoglutarate on Escherichia coli metabolism.

ABSTRACT: Desmethylphosphinothricin (L-Glu-γ-P_H) is the H-phosphinic analog of glutamate with carbon-phosphorus-hydrogen (C-P-H) bonds. In L-Glu-γ-P_H the phosphinic group acts as a bioisostere of the glutamate γ-carboxyl group allowing the molecule to be a substrate of Escherichia coli glutamate decarboxylase, a pyridoxal 5'-phosphate-dependent α-decarboxylase. In addition, the L-Glu-γ-P_H decarboxylation product, GABA-P_H, is further metabolized by bacterial GABA-transaminase, another pyridoxal 5'-phosphate-dependent enzyme, and succinic semialdehyde dehydrogenase, a NADP⁺-dependent enzyme. The product of these consecutive reactions, the so-called GABA shunt, is succinate-P_H, the H-phosphinic analog of succinate, a tricarboxylic acid c

SUBMITTER: Giovannercole F 

PROVIDER: S-EPMC11533085 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature