Installation of an organocatalyst into a protein scaffold creates an artificial Stetterase.

ABSTRACT: Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst loading. Furthermore, activity can be increased >20 fold by altering the protein scaffold.

SUBMITTER: MacAulay A 

PROVIDER: S-EPMC11533139 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature