Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

ABSTRACT:

Background

It is widely accepted that glaucoma-induced oxidative stress expedites cataracts' process. Therefore, we examined the effects of apelin-13 against oxidative stress-induced damage in human lens epithelial cells (HLECs) and investigated the potential pathogenic mechanism of acute primary angle-closure glaucoma.

Methods

This experiment included five groups: control, H₂O₂, apelin-13 + H₂O₂, ML221 + H₂O₂, and apelin-13 + ML221 + H₂O₂. ML221 was employed in rescue experiments as an APJ antagonist. HLECs were pretreated with or without apelin-13 and subsequently exposed to H₂O₂. HLECs' viability was assessed by CCK8. Cell apoptosis was determined using Annexin V-

SUBMITTER: Li X 

PROVIDER: S-EPMC11533313 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature