A yeast two-hybrid system to obtain triple-helical ligands from combinatorial random peptide libraries.

ABSTRACT: Many bioactive proteins interact with collagen, recognizing amino acid sequences displayed on the triple helix. We report here a selection strategy to obtain triple-helical peptides that interact with the proteins from a combinatorial random library constructed in yeast cells. This system enables us to select them using the standard two-hybrid protocol, detecting interactions between triple-helical peptides and target proteins fused to the GAL4-activating and binding domains, respectively. The library was constructed having triple-helical peptides with a "host-guest" design in which host helix-stabilizing regions flanked guest random sequences. Using this system, we selected peptides that bind to pigment epithelium-derived factor (PEDF), a collagen-binding protein that shows anti-angiogeni

SUBMITTER: Masuda R 

PROVIDER: S-EPMC11533527 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature