Cigarette smoke extract induces ferroptosis in human retinal pigment epithelial cells.

ABSTRACT:

Background

Age-related macular degeneration (AMD) is a common blindness diseases. Retinal pigment epithelium (RPE) dysfunction due to smoking is an essential environmental factor in the pathogenesis of AMD. Ferroptosis is a novel type of iron-dependent programmed cell death (PCD). However, the relationship between cigarette smoke extract (CSE)-induced RPE damage and ferroptosis remains unclear.

Methods

In our study, we extracted CSE using a modified device to explore the optimal concentration of CSE, and observed the expression of proteins and molecules after CSE exposure for ARPE-19 cells by protein immunoblotting and assay kits for iron ions and mitochondrial membrane potential (MMP). At the same time, CSE was injected into the vitreous cavity of mice with a microsyringe f

SUBMITTER: Zhao L 

PROVIDER: S-EPMC11533555 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature