Fth1-mScarlet Reports Monocyte State during Lipopolysaccharide-induced Lung Inflammation.

ABSTRACT: Monocytes and macrophages are central to host defense but also contribute to inflammation-associated pathology. Efforts to manipulate monocyte and macrophage function are limited by our ability to effectively quantify the functional programs of these cells. We identified the gene Fth1, which encodes the ferritin H chain, as highly predictive of alveolar macrophage transcriptomic states during LPS-induced lung inflammation and developed an Fth1-mScarlet reporter mouse. In the steady-state lung, high Fth1-mScarlet expression is restricted to alveolar macrophages. In response to LPS-induced lung inflammation, Fth1 reporter activity is robustly increased in monocytes, with its expression reporting genes that are differentially expressed in monocytes versus macrophages. Consistent with this rep

SUBMITTER: Michalides BA 

PROVIDER: S-EPMC11534542 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature