Protective effect of gut microbiota restored by fecal microbiota transplantation in a sepsis model in juvenile mice.

ABSTRACT:

Introduction

Restoring a balanced, healthy gut microbiota through fecal microbiota transplantation (FMT) has the potential to be a treatment option for sepsis, despite the current lack of evidence. This study aimed to investigate the effect of FMT on sepsis in relation to the gut microbiota through a sepsis model in juvenile mice.

Methods

Three-week-old male mice were divided into three groups: the antibiotic treatment (ABX), ABX-FMT, and control groups. The ABX and ABX-FMT groups received antibiotics for seven days. FMT was performed through oral gavage in the ABX-FMT group over the subsequent seven days. On day 14, all mice underwent cecal ligation and puncture (CLP) to induce abdominal sepsis. Blood cytokine levels and the composition of fecal microbiota were analyzed, an

SUBMITTER: Han YJ 

PROVIDER: S-EPMC11534669 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature