Mitochondrial DNA content and methylation in sperm of patients with asthenozoospermia.

ABSTRACT:

Purpose

The aim of the current study was to investigate the mtDNA methylation levels and mtDNA copy numbers in the sperm of patients with asthenozoospermia and compare them to those observed in controls with normozoospermia.

Methods

Pyrosequencing analysis of the methylation levels of the mitochondrial D-loop and MT-CO1/chr1:631,907-632083/chrX:26,471,887-126,472,063 (hereinafter referred to as "MT-CO1-AVG") region and quantitative PCR analysis of the mtDNA copy number were performed on sperm from 30 patients with asthenozoospermia and 30 controls with normozoospermia.

Results

Compared with those of controls with normozoospermia, the methylation levels of D-loop and MT-CO1-AVG regions and mtDNA copy number were significantly higher in patients with asthenozoospermia.

SUBMITTER: Geng Q 

PROVIDER: S-EPMC11535106 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature