Probing Glycerolipid Metabolism using a Caged Clickable Glycerol-3-Phosphate Probe.

ABSTRACT: In this study, we present the probe SATE-G3P-N₃ as a novel tool for metabolic labeling of glycerolipids (GLs) to investigate lipid metabolism in yeast cells. By introducing a clickable azide handle onto the glycerol backbone, this probe enables general labeling of glycerolipids. Additionally, this probe contains a caged phosphate moiety at the glycerol sn-3 position to not only facilitate probe uptake by masking negative charge but also to bypass the phosphorylation step crucial for initiating phospholipid synthesis, thereby enhancing phospholipid labeling. The metabolic labeling activity of the probe was thoroughly assessed through cellular fluorescence microscopy, mass spectrometry (MS), and thin-layer chromatography (TLC) experiments. Fluorescence microscopy analysis demonstr

SUBMITTER: Lou J 

PROVIDER: S-EPMC11535253 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature