Toe1 promotes proliferation and differentiation of neural progenitor cells.

ABSTRACT: Toe1 (target of EGR1, member 1) is a 3'- exonuclease in the deadenylase family, essential for the maturation of small nuclear RNAs. Mutations in Toe1 are linked to pontocerebellar hypoplasia 7 (PCH7), a severe neurodegenerative syndrome affecting infants, characterized by progressive neurodegeneration, developmental delay, and genital abnormalities. The pathogenic mechanisms of PCH7 are unclear but are thought to involve abnormal neural stem cell (NSC) development during embryogenesis. This study investigates Toe1's role in NSC development using the C17.2 NSC line. Colony formation, EdU incorporation, and CFSE staining assays showed that Toe1 knockout inhibited C17.2 cell proliferation. Upon inducing differentiation, Toe1 knockout significantly reduced cell dendrites. Immunofluorescence, q

SUBMITTER: Deng T 

PROVIDER: S-EPMC11535345 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature