SEV-mediated lipid droplets transferred from bone marrow adipocytes promote ferroptosis and impair osteoblast function.

ABSTRACT: Osteoporosis is linked to increased bone marrow adipocyte (BMAd) proliferation, which displaces bone-forming cells and alters the local environment. The impact of BMAd lipid droplets on bone health and osteoblast function remains unclear. This study investigates the interplay between BMAd-derived lipid droplets and osteoblast functionality, focusing on ferroptosis pathways. Osteoblast cultures were treated with conditioned media from adipocytes to simulate in vivo conditions. High-throughput mRNA sequencing and Western blot analysis were used to profile changes in gene expression and protein levels related to ferroptosis, oxidative phosphorylation, and osteogenic markers. Cellular assays assessed the direct impact of lipid droplets on osteoblast activity. Results showed that osteoblasts ex

SUBMITTER: Huang W 

PROVIDER: S-EPMC11535364 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature