Pharmacological Characterization of the Endocannabinoid Sensor GRAB_eCB2.0.

ABSTRACT: Introduction: The endocannabinoids (eCBs), 2-arachidonoylglycerol (2-AG) and arachidonoyl ethanolamine (AEA), are produced by separate enzymatic pathways, activate cannabinoid (CB) receptors with distinct pharmacological profiles, and differentially regulate pathophysiological processes. The genetically encoded sensor, GRAB_eCB2.0, detects real-time changes in eCB levels in cells in culture and preclinical model systems; however, its activation by eCB analogues produced by cells and by phyto-CBs remains uncharacterized, a current limitation when interpreting changes in its response. This information could provide additional utility for the tool in in vivo pharmacology studies of phyto-CB action. Materials and Methods: GRAB_eCB2.0 was expressed in cu

SUBMITTER: Singh S 

PROVIDER: S-EPMC11535446 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature