Systematic mapping of mitochondrial calcium uniporter channel (MCUC)-mediated calcium signaling networks.

ABSTRACT: The mitochondrial calcium uniporter channel (MCUC) mediates mitochondrial calcium entry, regulating energy metabolism and cell death. Although several MCUC components have been identified, the molecular basis of mitochondrial calcium signaling networks and their remodeling upon changes in uniporter activity have not been assessed. Here, we map the MCUC interactome under resting conditions and upon chronic loss or gain of mitochondrial calcium uptake. We identify 89 high-confidence interactors that link MCUC to several mitochondrial complexes and pathways, half of which are associated with human disease. As a proof-of-concept, we validate the mitochondrial intermembrane space protein EFHD1 as a binding partner of the MCUC subunits MCU, EMRE, and MCUB. We further show a MICU1-dependent inhib

SUBMITTER: Delgado de la Herran H 

PROVIDER: S-EPMC11535509 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature