Endosialin-positive CAFs promote hepatocellular carcinoma progression by suppressing CD8⁺ T cell infiltration.

ABSTRACT:

Background and aims

Endosialin, also known as tumor endothelial marker1 or CD248, is a transmembrane glycoprotein that is mainly expressed in cancer-associated fibroblasts (CAFs) in hepatocellular carcinoma (HCC). Our previous study has found that endosialin-positive CAFs could recruit and induce the M2 polarization of macrophages in HCC. However, whether they may regulate other types of immune cells to promoting HCC progression is not known.

Approach and results

The growth of both subcutaneous and orthotopic HCC tumors was significantly inhibited in endosialin knockout (EN^KO) mice. Single-cell sequencing and flow cytometry analysis showed that tumor tissues from EN^KO mice had increased CD8⁺ T cell infiltration. Mixed HCC tumor with Hepa1-6 c

SUBMITTER: Gan L 

PROVIDER: S-EPMC11535718 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature