Mammalian D-Cysteine controls insulin secretion in the pancreas.

ABSTRACT:

Background

D-amino acids are being recognized as important molecules in mammals with function. This is a first identification of endogenous D-cysteine in mammalian pancreas.

Methods

Using a novel stereospecific bioluminescent assay, chiral chromatography, enzyme kinetics and a transgenic mouse model we identify endogenous D-cysteine. We elucidate its function in two mice models of type 1 diabetes (STZ and NOD), and in tests of Glucose Stimulated Insulin Secretion in isolated mouse and human islets and INS-1 832/13 cell line.

Results and discussion

D-cysteine is synthesized by serine racemase (SR) and SR^-/- mice produce 6-10 fold higher levels of insulin in the pancreas and plasma including higher glycogen and ketone bodies in the liver. The excess insulin i

SUBMITTER: Roychaudhuri R 

PROVIDER: S-EPMC11536007 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature