OBP2A regulates epidermal barrier function and protects against cytotoxic small hydrophobic molecules.

ABSTRACT: The skin is constantly exposed to environmental sensory stimuli, which may include harmful volatiles and small hydrophobic molecules. However, the skin's protective mechanism against the latter agents is unclear. Here, we demonstrate that odorant binding protein 2A (OBP2A) protects epidermal keratinocytes against cytotoxic small hydrophobic molecules. OBP2A is mainly expressed in human epidermal keratinocytes. Cellular resistance to cytotoxic aldehyde and lipids was reduced in keratinocytes when OBP2A was silenced. Furthermore, silencing of OBP2A in a three-dimensional epidermal equivalent model resulted in impairment of epidermal barrier function. Inhibition of OBP2A caused disruption of keratinocyte lipid metabolism and induced endoplasmic reticulum stress. OBP2A expression was markedly

SUBMITTER: Nakanishi S 

PROVIDER: S-EPMC11536036 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature