Cryo-EM structure of the zinc-activated channel (ZAC) in the Cys-loop receptor superfamily.

ABSTRACT: Cys-loop receptors are a large superfamily of pentameric ligand-gated ion channels with various physiological roles, especially in neurotransmission in the central nervous system. Among them, zinc-activated channel (ZAC) is a Zn²⁺-activated ion channel that is widely expressed in the human body and is conserved among eukaryotes. Due to its gating by extracellular Zn²⁺, ZAC has been considered a Zn²⁺ sensor, but it has undergone minimal structural and functional characterization since its molecular cloning. Among the families in the Cys-loop receptor superfamily, only the structure of ZAC has yet to be determined. Here, we determined the cryo-EM structure of ZAC in the apo state and performed structure-based mutation analyses. We identified a few residues in

SUBMITTER: Jin F 

PROVIDER: S-EPMC11536092 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature