Differential Localization and Functional Roles of mGluR6 Paralogs in Zebrafish Retina.

ABSTRACT:

Purpose

To define the location of mglur6 paralogs in the outer zebrafish retina and delineate their contribution to retina light responses across the visual spectrum.

Methods

In situ hybridization and immunolocalization with custom-made antibodies were used to localize mglur6 transcripts, proteins, and additional components of the mGluR6 signaling complex. Gene editing was used to generate knockout mutants that were analyzed with white light and spectral electroretinography.

Results

Both mglur6 paralogs colocalized with known downstream pathway genes, such as trpm1a, nyctalopin, and gnaoβ. All rod photoreceptors contacted mGluR6-positive cells, while cone connectivity presented a more complex situation with no red cones and only a few UV and blue-sensitive cones conne

SUBMITTER: Haug M 

PROVIDER: S-EPMC11536201 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature