Gene discovery from microbial gene libraries I: protection against reactive oxygen species-driven DNA damage.

ABSTRACT: Reactive oxygen species (ROS) pose a lethal risk for all life forms by causing damage to cell processes, genome-wide DNA damage-driving mutation, replicative instability, and death. Thus, the development of mechanisms to resist or repair ROS-induced DNA damage is critical for the reliable replication of nucleic acids. DNA repair and protection mechanisms have been discovered in all forms of life. However, the vast array of microbes that may harbor novel repair or protection mechanisms, especially bacterial viruses, have not been adequately assessed. Here, we screened a microbial gene library composed primarily of phage open reading frames (ORFs) to uncover elements that overcome a DNA damage blockade. We report the discovery of one such protein, termed F21, which promotes bacterial surviva

SUBMITTER: Taylor JC 

PROVIDER: S-EPMC11536983 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature