Modular, inducible, and titratable expression systems for <i>Escherichia coli</i> and <i>Acinetobacter baumannii</i>.

ABSTRACT: Gene expression systems that transcend species barriers are needed for cross-species analysis of gene function. In particular, expression systems that can be utilized in both model and pathogenic bacteria underpin comparative functional approaches that inform conserved and variable features of bacterial physiology. In this study, we develop replicative and integrative vectors alongside a novel, IPTG-inducible promoter that can be used in the model bacterium Escherichia coli K-12 as well as strains of the antibiotic-resistant pathogen, Acinetobacter baumannii. We generate modular vectors that transfer by conjugation at high efficiency and either replicate or integrate into the genome, depending on design. Embedded in these vectors, we also developed a synthetic, IPTG-inducible

SUBMITTER: Bacon EE 

PROVIDER: S-EPMC11536989 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature