Coupling antitoxins and blue/white screening with <i>parAB</i>/resolvase mutation as a strategy for <i>Salmonella</i> spp. plasmid curing.

ABSTRACT: Despite the dissemination of multidrug resistance plasmids, including those carrying virulence genes in Salmonella spp., efficient plasmid curing tools are lacking. Plasmid partitioning and multimer resolution systems are attractive targets for plasmid cure. However, plasmid curing strategies targeting these systems are often hindered by the host addiction system through a process known as post-segregation killing. Here, we developed vector tools that can mutate the above systems while replenishing short-lived antitoxins. Cloning was performed using Gibson assembly. parAB or resolvase (res) genes on Incompatibility Group (Inc)FIB, IncA/C, IncX4, and plasmids carried by Salmonella species were deleted by first knocking in the N-terminal ß-galactosidase encoding g

SUBMITTER: Gudeta DD 

PROVIDER: S-EPMC11537010 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature