Direct genome sequencing of respiratory viruses from low viral load clinical specimens using the target capture sequencing technology.

ABSTRACT: The use of metagenomic next-generation sequencing technology to obtain complete viral genome sequences directly from clinical samples with low viral load remains challenging-especially in the case of respiratory viruses-due to the low copy number of viral versus host genomes. To overcome this limitation, target capture sequencing for the enrichment of specific genomes has been developed and applied for direct genome sequencing of viruses. However, as the efficiency of enrichment varies depending on the probes, the type of clinical sample, etc., validation is essential before target capture sequencing can be applied to clinical diagnostics. In this study, we evaluated the utility of target capture sequencing with a comprehensive viral probe panel for clinical respiratory specimens collected

SUBMITTER: Takemae N 

PROVIDER: S-EPMC11537015 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature