Acidocalcisome localization of membrane transporters and enzymes in <i>Trypanosoma brucei</i>.

ABSTRACT: Acidocalcisomes of Trypanosoma brucei are membrane-bounded organelles characterized by their acidity and high content of polyphosphate and cations, like calcium and magnesium. They have important roles in cation and phosphorus storage, osmoregulation, autophagy initiation, calcium signaling, and virulence. Acidocalcisomes of T. brucei possess several membrane transporters, pumps, and channels, some of which were identified by proteomic and immunofluorescence analyses and validated as acidocalcisome proteins by their colocalization with the acidocalcisome marker vacuolar proton pyrophosphatase (VP1). Here, we report that a set of membrane transporters and enzymes, which were proposed to be present in acidocalcisomes by the morphological appearance of tagged proteins, colocaliz

SUBMITTER: Huang G 

PROVIDER: S-EPMC11537032 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature