Near-infrared <i>in vivo</i> imaging system for dynamic visualization of lung-colonizing bacteria in mouse pneumonia.

ABSTRACT: In vivo imaging of bacterial infection models enables noninvasive and temporal analysis of individuals, enhancing our understanding of infectious disease pathogenesis. Conventional in vivo imaging methods for bacterial infection models involve the insertion of the bacterial luciferase LuxCDABE into the bacterial genome, followed by imaging using an expensive ultrasensitive charge-coupled device (CCD) camera. However, issues such as limited light penetration into the body and lack of versatility have been encountered. We focused on near-infrared (NIR) light, which penetrates the body effectively, and attempted to establish an in vivo imaging method to evaluate the number of lung-colonizing bacteria during the course of bacterial pneumonia. This was achieved by employing

SUBMITTER: Yamaguchi D 

PROVIDER: S-EPMC11537041 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature