Cell wall nanoparticles from hyphae of <i>Alternaria infectoria</i> grown with caspofungin, nikkomycin, or pyroquilon trigger different activation profiles in macrophages.

ABSTRACT: Alternaria infectoria causes opportunistic human infections and is a source of allergens leading to respiratory allergies. In this work, we prepared cell wall nanoparticles (CWNPs) as a novel approach to study macrophage immunomodulation by fungal hyphal cell walls. A. infectoria was grown in the presence of caspofungin, an inhibitor of β(1,3)-glucan synthesis; nikkomycin Z, an inhibitor of chitin synthases; and pyroquilon, an inhibitor of dihydroxynaphthalene (DHN)-melanin synthesis. Distinct CWNPs were obtained from these cultures, referred to as casCWNPs, nkCWNPs, and pyrCWNPs, respectively. CWNPs are round-shaped particles with a diameter of 70-200 nm diameter particles that when added to macrophages are taken up by membrane ruffling. CWNPs with no DHN-melanin and more gl

SUBMITTER: Antunes D 

PROVIDER: S-EPMC11537108 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature