Assessment of optimized FRET substrates as universal corona- and picornavirus main protease substrates for screening assays.

ABSTRACT: Coronaviral infections are an important cause of enteric and respiratory diseases in humans and animals that are generally associated with a high level of morbidity and mortality. Similarly, picornavirus infections can lead to various illnesses that severely impact human and animal health. Despite belonging to different virus families, viral replication in all of these pathogens relies on the action of a central cysteine protease called 3C/3CL or main protease (M^pro). Due to the high functional and structural conservation of this enzyme among viral species and robustness against mutation it is considered a good target for antiviral inhibitor development. The evaluation of inhibitor potency, expressed as IC₅₀, in many studies is achieved by measuring the inhibition of

SUBMITTER: Fischer C 

PROVIDER: S-EPMC11537199 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature