Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila.

ABSTRACT: A previously described sequence-based epidemiological typing method for clinical and environmental isolates of Legionella pneumophila serogroup 1 was extended by the investigation of three additional gene targets and modification of one of the previous targets. Excellent typeability, reproducibility, and epidemiological concordance were determined for isolates belonging to both serogroup 1 and the other serogroups investigated. Gene fragments were amplified from genomic DNA, and PCR amplicons were sequenced by using forward and reverse primers. Consensus sequences are entered into an online database, which allows the assignment of individual allele numbers. The resulting sequence-based type or allelic profile comprises a string of the individual allele numbers separated by commas, e.g., 1,

SUBMITTER: Gaia V 

PROVIDER: S-EPMC1153775 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature