FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics.

ABSTRACT: Rare cells have an important role in development and disease, and methods for isolating and studying cell subsets are therefore an essential part of biology research. Such methods traditionally rely on labeled antibodies targeted to cell surface proteins, but large public databases and sophisticated computational approaches increasingly define cell subsets on the basis of genomic, epigenomic and transcriptomic sequencing data. Methods for isolating cells on the basis of nucleic acid sequences powerfully complement these approaches by providing experimental access to cell subsets discovered in cell atlases, as well as those that cannot be otherwise isolated, including cells infected with pathogens, with specific DNA mutations or with unique transcriptional or splicing signatures. We recentl

SUBMITTER: Shin SW 

PROVIDER: S-EPMC11537836 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature