Activation of estrogen-related receptor γ by calcium and cadmium.

ABSTRACT:

Objective

Estrogen-related receptor γ (ERRγ) is a metabolic regulator with no identified physiological ligands. This study investigates whether calcium is an ERRγ ligand that mediates the effects of glucagon and whether cadmium, which mimics the effects of calcium, disrupts metabolism through ERRγ.

Method

HepG2, MCF-7, and HEK293T transfected with ERRγ were treated with glucagon, calcium, cadmium, ERRγ agonist, or ERRγ inhibitor. Cells were then collected for in vitro assays including real-time qPCR, Western blot, ChIP, immunofluorescence, mutational analysis, or gene set enrichment analysis. Molecular dynamics simulations were performed to study mutation sites.

Results

In HepG2 cells, treatment with glucagon, calcium, or cadmium re-localized ERRγ to the cell n

SUBMITTER: Wang Q 

PROVIDER: S-EPMC11537906 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature