A rapid and visual detection assay for Senecavirus A based on recombinase-aided amplification and lateral flow dipstick.

ABSTRACT:

Background

Senecavirus A (SVA) is a newly pathogenic virus correlated with the acute death of piglets and vesicular lesions in pigs. The further prevalence of SVA will cause considerable economic damage to the global pig farming industry. Therefore, rapid and accurate diagnostic tools for SVA are crucial for preventing and controlling the disease.

Methods

We designed multiple primer pairs targeting the most conserved region of the SVA 3D gene and selected those with the highest specificity. Nfo-probes were subsequently developed based on the highest specificity primer pairs. Subsequently, the recombinase-assisted amplification (RAA) reaction was completed, and the reaction temperature and duration were optimized. The RAA amplicons were detected using a lateral flow de

SUBMITTER: Song Y 

PROVIDER: S-EPMC11538013 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature