A yeast surface display platform for screening of non-enzymatic protein secretion in Kluyveromyces lactis.

ABSTRACT: Enhancing the secretion of recombinant proteins, particularly non-enzymatic proteins that predominate in food and pharmaceutic protein products, remains a significant challenge due to limitations in high-throughput screening methods. This study addresses this bottleneck by establishing a yeast surface display system in the food-grade microorganism Kluyveromyces lactis, enabling efficient display of model target proteins on the yeast cell surface. To assess its potential as a universal high-throughput screening tool for enhanced non-enzymatic protein secretion, we evaluated the consistency between protein display levels and secretion efficiency under the influence of various genetic factors. Our results revealed a strong correlation between these two properties. Furthermore, screening in a

SUBMITTER: An J 

PROVIDER: S-EPMC11538148 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature