A New Bacterial Chassis for Enhanced Surface Display of Recombinant Proteins.

ABSTRACT:

Introduction

Bacterial surface display is a valuable biotechnology technique for presenting proteins and molecules on the outer surface of bacterial cells. However, it has limitations, including potential toxicity to host bacteria and variability in display efficiency. To address these issues, we investigated the removal of abundant non-essential outer membrane proteins (OMPs) in E. coli as a new strategy to improve the surface display of recombinant proteins.

Methods

We targeted OmpA, a highly prevalent OMP in E. coli, using the lambda red method. We successfully knocked out ompA in two E. coli strains, K-12 MG1655 and E. coli BL-21, which have broad research and therapeutic applications. We then combined ompA knockout strains and two OMPs

SUBMITTER: Zhang R 

PROVIDER: S-EPMC11538204 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature