Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme.

ABSTRACT: The emergence of multidrug-resistant Klebsiella pneumoniae poses significant clinical challenges with limited treatment options. Biofilm is an important virulence factor of K. pneumoniae, serving as a protective barrier against antibiotics and the immune system. Here, we present the remarkable ability of a bovine microbial enzyme to prevent biofilm formation (IC₅₀ 2.50 μM) and degrade pre-formed K. pneumoniae biofilms (EC₅₀ 1.94 μM) by degrading the matrix polysaccharides. The treatment was effective against four different clinical K. pneumoniae isolates tested. Moreover, the enzyme significantly improved the biofilm sensitivity of a poorly performing broad-spectrum antibiotic, meropenem, and immune cells, resulting in facile biofilm clearance from the mouse wound inf

SUBMITTER: Ramakrishnan R 

PROVIDER: S-EPMC11538315 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature