Characterization and functional evaluation of goat PDX1 regulatory modules through comparative analysis of conserved interspecies homologs.

ABSTRACT: PDX1 is a crucial transcription factor in pancreas development and mature β-cell function. However, the regulation of PDX1 expression in larger animals mirroring human pancreas morphogenesis and endocrine maturation remains poorly understood. Therefore, we conducted a comparative analysis to characterize regulatory regions of goat PDX1 gene and assessed their transcriptional activity by transient transfection of several transgenic EGFP constructs in β- and non-β cell lines. We recognized several highly conserved regions encompassing the promoter and cis-regulatory elements (Area I-IV) at 5' flanking sequence of the genes. Within the promoter, we identified that a key E-box and nearby CAAT element synergistically drive transcription, constituting the basal promoter of goat PDX1 gene. Furthe

SUBMITTER: Rezaei N 

PROVIDER: S-EPMC11538457 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature