Electro-Driven Multi-Enzymatic Cascade Conversion of CO₂ to Ethylene Glycol in Nano-Reactor.

ABSTRACT: Multi-enzymatic cascade reaction provides a new avenue for C─C coupling directly from CO₂ under mild conditions. In this study, a new pathway with four enzymes including formate dehydrogenase (PaFDH), formaldehyde dehydrogenase (BmFADH), glycolaldehyde synthase (PpGALS), and alcohol dehydrogenase (GoADH) is developed for directly converting CO₂ gas molecules to ethylene glycol (EG) in vitro. A rhodium-based NADH regeneration electrode is constructed to continuously provide the proton and electron of this multi-enzymatic cascade reaction. The prepared electrode can reach the Faradaic Efficiency (FE) of 82.9% at -0.6 V (vs. Ag/AgCl) and the NADH productivity of 0.737 mM h^-1. Shortening the reaction path is crucial for multi-enzymatic cascade reactions. Here,

SUBMITTER: Luan L 

PROVIDER: S-EPMC11538636 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature