Isobutyric Acid Promotes Immune Evasion in Colorectal Cancer via Increased PD-L1 Expression.

ABSTRACT:

Introduction

Isobutyric acid (IBA), a short-chain fatty acid, has been unequivocally demonstrated to exert significant influence on the progression of colorectal cancer (CRC). Nevertheless, a comprehensive understanding of its intricate regulatory mechanisms remains elusive.

Methods

Employing advanced techniques such as western blot, RT-qPCR, and flow cytometry, we systematically investigated the impact of IBA on the expression of PD-L1 in CRC cells. Concurrently, employing RNA silencing technology and small-molecule inhibitors, we delved into the molecular intricacies underlying the regulatory axis of IBA involving ROCK1/c-Myc/PD-L1. Furthermore, through flow cytometry analysis, we examined the alterations in the tumor immune microenvironment following anti-PD-L1 antibody t

SUBMITTER: Lin Q 

PROVIDER: S-EPMC11538990 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature