Spatial heterogeneity analysis of seeding of human induced pluripotent stem cells for neuroectodermal differentiation.

ABSTRACT:

Introduction

Preparing a uniform cell population in high-density seeding of adherent human induced pluripotent stem cells (hiPSC) requires stable culture conditions and consistent culture operation. In this study, we evaluated cell distribution patterns by changing cell seeding operations and their impact on differentiation toward the neuroectodermal lineage.

Methods

The hiPSC line 201B7 was seeded at 1.23 × 10⁵ cells/cm² following a conventional operation, prolongated time of cell seeding suspension or vessel tilting during cell seeding operation. Fluorescent imaging of cell nuclei was performed 24 h following cell seeding and used for spatial heterogeneity analysis. Flow cytometric analysis was also performed seven days after cell differentiation indu

SUBMITTER: Qatan AAI 

PROVIDER: S-EPMC11539164 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature