Shining a light on the impact of antifungals on <i>Aspergillus fumigatus</i> subcellular dynamics through fluorescence imaging.

ABSTRACT: Fluorescent proteins (FPs) are indispensable tools used for molecular imaging, single-cell dynamics, imaging in infection models, and more. However, next-generation FPs have yet to be characterized in Aspergillus. Here, we characterize 18 FPs in the pathogenic filamentous fungus Aspergillus fumigatus spanning the visible light spectrum. We report on in vivo FP brightness in hyphal and spore morphotypes and show how a fluoropyrimidine-based selection system can be used to iteratively introduce four distinct FPs enabling the simultaneous visualization of the cell membrane, mitochondria, peroxisomes, and vacuoles. Using this strain, we describe and compare the dynamic responses of organelles to stresses induced by voriconazole, amphotericin B, and the novel antifungal dru

SUBMITTER: Storer ISR 

PROVIDER: S-EPMC11539212 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature