Sodium arsenite-induced DNA methylation alterations exacerbated by p53 knockout in MCF7 cells.

ABSTRACT: Epigenetic alterations are ubiquitous across human malignancies. Thus, functional characterization of epigenetic events deregulated by environmental pollutants should enhance our understanding of the mechanisms of carcinogenesis and inform preventive strategies. Recent reports showing the presence of known cancer-driving mutations in normal tissues have sparked debate on the importance of non-mutational stressors potentially acting as cancer promoters. Here, we aimed to test the hypothesis that the presence of mutations in p53, a commonly mutated gene in human malignancies, may influence cellular response to an environmental non-mutagenic agent, potentially involving epigenetic mechanism. We used the CRISPR-Cas9 system to generate knockouts of p53 in MCF7 and T47D breast cancer cell lines

SUBMITTER: Chung FF 

PROVIDER: S-EPMC11539298 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature