Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems.

ABSTRACT:

Background

De-novo-designed synthetic transcriptional regulators have great potential as the genetic parts for constructing complex multilayered gene circuits. The design flexibility afforded by advanced nucleic acid sequence design tools vastly expands the repertoire of regulatory elements for circuit design. In principle, the design space of synthetic regulators should allow for the construction of regulatory circuits of arbitrary complexity; still, the orthogonality and robustness of such components have not been fully elucidated, thereby limiting the depth and width of synthetic circuits.

Results

In this work, we systematically explored the design strategy of synthetic transcriptional regulators, termed switchable transcription terminators. Specifically, by redesigning k

SUBMITTER: Zhao M 

PROVIDER: S-EPMC11539451 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature