Single cell RNA-seq reveals cellular and transcriptional heterogeneity in the splenic CD11b⁺Ly6C^high monocyte population expanded in sepsis-surviving mice.

ABSTRACT:

Background

Sepsis survivors exhibit immune dysregulation that contributes to poor long-term outcomes. Phenotypic and functional alterations within the myeloid compartment are believed to be a contributing factor. Here we dissect the cellular and transcriptional heterogeneity of splenic CD11b⁺Ly6C^high myeloid cells that are expanded in mice that survive the cecal ligation and puncture (CLP) murine model of polymicrobial sepsis to better understand the basis of immune dysregulation in sepsis survivors.

Methods

Sham or CLP surgeries were performed on C57BL/6J and BALB/c mice. Four weeks later splenic CD11b⁺Ly6C^high cells from both groups were isolated for phenotypic (flow cytometry) and functional (phagocytosis and glycolysis) charact

SUBMITTER: Watanabe H 

PROVIDER: S-EPMC11539566 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature