SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons.

ABSTRACT: Inhibitory synaptic neurotransmission mediated by GABA requires a low concentration of chloride ions (Cl^-) in neurons, which is established and maintained by the potassium-chloride co-transporter 2 (KCC2). While KCC2-interacting proteins are known to regulate KCC2 protein level and function, specific KCC2-interacting partners are still being identified and characterized. We asked whether SNAP25, an integral component of the SNARE-complex and a novel KCC2 interactor, regulates KCC2 protein and function in mice. We demonstrated that SNAP25 interacts with KCC2, and that this interaction is regulated by protein kinase C (PKC)-mediated phosphorylation. We also discovered that SNAP25 knockdown decreases total KCC2 in cortical neurons, and reduces the strength of synaptic inhibition, a

SUBMITTER: Raveendran VA 

PROVIDER: S-EPMC11539599 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature