Relationships between HDL subpopulation proteome and HDL function in overweight/obese people with and without coronary heart disease.

ABSTRACT:

Background and aims

The structure-function relationships of high-density lipoprotein (HDL) subpopulations are not well understood. Our aim was to examine the interrelationships between HDL particle proteome and HDL functionality in subjects with and without coronary heart disease (CHD).

Methods

We isolated 5 different HDL subpopulations based on charge, size, and apolipoprotein A1 (APOA1) content from the plasma of 33 overweight/obese CHD patients and 33 age-and body mass index (BMI)-matched CHD-free subjects. We measured the relative molar concentration of HDL-associated proteins by liquid chromatography tandem mass spectrometry (LC-MS/MS) and assessed particle functionality.

Results

We quantified 110 proteins associated with the 5 APOA1-containing HDL subpopulations

SUBMITTER: Vaisar T 

PROVIDER: S-EPMC11539851 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature