Regulatory mechanism of cysteine-dependent methionine biosynthesis in <i>Bifidobacterium longum</i>: insights into sulfur metabolism in gut microbiota.

ABSTRACT: Sulfur, a critical element for bacterial growth, is not directly utilized by bifidobacteria, rendering the sulfur-containing amino acid biosynthesis pathway, particularly for cysteine and methionine, poorly understood. This research identifies six genes involved in this pathway through re-annotation of the Bifidobacterium longum DJO10A genome. These genes play crucial roles in bioconversion processes essential for cysteine utilization, highlighting its significance in sulfur metabolism. Our study uncovers a novel regulatory mechanism of these pathways under varying cysteine concentrations. We demonstrate a dual-pathway mechanism for methionine biosynthesis: one directly utilizing cysteine (trans-sulfurylation pathway) and another utilizing H₂S derived from cysteine degrad

SUBMITTER: Kim YT 

PROVIDER: S-EPMC11540073 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature