Regional immune mechanisms enhance efficacy of an autologous cellular cancer vaccine with intraperitoneal administration.

ABSTRACT: Widespread peritoneal dissemination is common in patients with gynecologic or gastrointestinal cancers. Accumulating evidence of a central role for regional immunity in cancer control indicates that intraperitoneal immunotherapy may have treatment advantages. This study delineates immune mechanisms engaged by intraperitoneal delivery of a cell-based vaccine comprised of silicified ovarian cancer cells associated with enhanced survival. Vaccine trafficking from the site of injection to milky spots and other fat-associated lymphoid clusters was studied in syngeneic cancer models using bioluminescent and fluorescent imaging, microscopy, and flow cytometry. Spectral flow cytometry was used to phenotype peritoneal immune cell populations, while bioluminescent imaging of cancer was used to study

SUBMITTER: Marwedel B 

PROVIDER: S-EPMC11540083 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature