Detection of DNA methylation from buccal swabs using nanopore sequencing to study stunting.

ABSTRACT: Stunting is the result of chronic malnutrition due to the lack of micronutrient-based methyl donors required for epigenetic programming during the first 1000 days of life. Methylation studies using bisulfite conversion from blood DNA are invasive and may not be practical for large-scale epidemiological investigation or nutrition intervention programs. Buccal epithelial methylation may reflect early germline methylation. Therefore, buccal cells can serve as convenient sample sources to collect biomarkers associated with the risk of stunting. This study aims to describe the feasibility of nanopore adaptive sampling in detecting DNA methylation from children's buccal DNA. We used adaptive sampling of Oxford Nanopore Technology on barcoded samples to describe differential methylation associate

SUBMITTER: El-Hakim A 

PROVIDER: S-EPMC11540103 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature