Tle6 deficiency in male mice led to abnormal sperm morphology and reduced sperm motility.

ABSTRACT: Infertility affects over 15% of the global population, and genetic mutations are a substantial cause of infertility. Recent studies have focused on the subcortical maternal complex and its role in early embryonic development. TLE6, a core protein in the subcortical maternal complex, is crucial for female fertility; however, its role in male germ cells remains poorly understood. In this study, we generated a novel Tle6 knockout mouse model using CRISPR-Cas9 to examine the impact of Tle6 mutations on male fertility. Tle6 knockout males exhibited a reduced total sperm count compared to wild-type mice, with a marked decrease in highly motile sperm. Histological observation of Tle6 ^+/- mouse testes showed no apparent structural changes, though impaired spe

SUBMITTER: Kazama K 

PROVIDER: S-EPMC11540623 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature