Stored RBC transfusions leads to the systemic inflammatory response syndrome in anemic murine neonates.

ABSTRACT:

Objective

RBC transfusions (RBCT) are life-saving treatment for premature and critically ill infants. However, the procedure has been associated with the development of systemic inflammatory response syndrome (SIRS) and potentially multiple organ dysfunction syndrome (MODS) in neonates. The present study aimed to investigate the mechanisms of RBCT-related SIRS in severely anemic murine neonates.

Methods

C57BL/6 (WT), TLR4^-/- and myeloid-specific triggered myeloid receptor-1 (trem1)^-/- mouse pups were studied in 4 groups (n = 6 each): (1) naïve controls, (2) transfused control, (3) anemic (hematocrit 20-24%) and (4) anemic with RBC transfused using our established murine model of phlebotomy-induced anemia (PIA) and RBC transfusion. Plasma was measured fo

SUBMITTER: Ramatchandirin B 

PROVIDER: S-EPMC11540732 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature