Enzymatic self-assembly of short peptides for cell spheroid formation.

ABSTRACT: Cell spheroids, including organoids, serve as a valuable link between in vitro systems and in vivo animal models, offering powerful tools for studying cell biology in a three-dimensional environment. However, existing methods for generating cell spheroids are time consuming or difficult to scale up for large-scale production. Our recent study has revealed that transcytotic peptide assemblies, which transform from nanoparticles to nanofibers by enzymatic reactions, can create an intercellular fibril/gel, accelerating cell spheroid formation from a 2D cell culture or a cell suspension. While this finding presents an alternative approach for generating cell spheroids, the specific structural features required for efficient cell spheroid formation remain unclear. Based on our obs

SUBMITTER: Guo J 

PROVIDER: S-EPMC11540748 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature