Structural basis of mRNA decay by the human exosome-ribosome supercomplex.

ABSTRACT: The interplay between translation and mRNA decay is widespread in human cells^1-3. In quality-control pathways, exonucleolytic degradation of mRNA associated with translating ribosomes is mediated largely by the cytoplasmic exosome^4-9, which includes the exoribonuclease complex EXO10 and the helicase complex SKI238 (refs. ^10-16). The helicase can extract mRNA from the ribosome and is expected to transfer it to the exoribonuclease core through a bridging factor, HBS1L3 (also known as SKI7), but the mechanisms of this molecular handover remain unclear^7,17,18. Here we reveal how human EXO10 is recruited by HBS1L3 (SKI7) to an active ribosome-bound SKI238 complex. We show that rather than a sequential handover, a direct physical coupling mechanism tak

SUBMITTER: Kogel A 

PROVIDER: S-EPMC11540850 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature